Update information

August, 2017
  • New categories, Fixed Specimen and Genotyping, were added to strain data.
  • Prices of resources were revised. (Refer to Price List for details)
  • We are now charging for Construct distribution. (Refer to Price List for details)
April, 2017 December, 2016
  • Evolution of the epidermis-specific expression of the gene encoding cellulose synthase of Ciona intestinalis was investigated with the genome editing technology. This provides a novel insight into the mechanisms of horizontal gene transfer. Click here for details.
April, 2016
  • A unique regulation of cell cycle compensation in the epidermal cells of Ciona neurula embryos was described. The regulation is essential for synchronous mitoses during embryogenesis. Many novel DNA constructs were used in this study, which have been added in this database. Click here for details.
November, 2015
  • The neuronal map of the central nervous system of Ciona juvenile was described in detail. Many transgenic lines in the NBRP project were used. Moreover, this manuscript provides the first report of the optogenetics in ascidian. Click here for details.
September 9, 2015 July, 2015
  • The function of Hox10 has been revealed in Ciona intestinalis. TALENs and enhancer trap lines of the NBRP were used in this study. Click here for details.
January, 2015
  • The international rules of the nomenclature of ascidian genetic resources (including transgenes and transgenic lines) were reported. Click here for details.
November 26, 2014 September, 2014
  • Knockout of Ciona intestinalis genes with Crispr/Cas9 was reported. This method will be breakthrough for analyzing gene functions in Ciona. Click here for details.
August, 2014
  • Transgenic lines expressing fluorescent cell cycle indicator (Fucci) probes were established. The transgenic lines were utilized to analyze the functions of GnRH peptides in Ciona. NBRP has started to deliver these Fucci lines. Click here for details.
July 1, 2014
  • Prices of resources were revised. (Refer to the Price List for the charges.)
May, 2014
  • In Ciona intestinalis, a novel technology named "MASK" that specifically knock-down maternal transcripts has been developed. This method will be breakthrough for analyzing molecular mechanisms during oogenesis. NBRP has started to deal with MASK transgenic lines in which Ci-pem and Ci-mT genes are knocked down. Click here for details.
April 4, 2014
  • Prices of resources were revised. (Refer to the Price List for the charges.)
March 6, 2014 January, 2014
  • Knockout of Ciona intestinalis with TALE nuclease was reported. Tissue-specific knockout of genes is capable with this method. TALEN-mediated mutagenesis will be breakthrough of analyzing gene functions in Ciona. Click here for details.
February 12, 2013 January 10, 2013
  • Strain data was added.
  • Construct data was added.
  • Publications page was updated.
  • Germline transgenesis of Ciona intestinalis with Sleeping Beauty transposon has been achieved. Click here for details.
  • A parasite seen in the culture system of Ciona intestinalis was reported in the Journal Diseases of Aquatic Organisms (vol 101, 185-195).
October 1, 2012
  • A mutant line of Hox1 gene has been published.
    This line is a powerful resource to study functions of Hox genes in Ciona intestinalis.
    This mutant line is available from our resource project.
  • ngs-news
    Fig: Hox1 mutant
  • Price List was updated.
  • Strain data was added.
  • Construct data was added.
  • Publications page was updated.
June 1, 2012
  • Gene targeting in Ciona intestinalis was reported. Click here for details.
February 6, 2012 April 4, 2011 August 19, 2010 August 2, 2010
  • A fee for strains will be charged. (Refer to the Price List for the charges.)
    Payment must be made by credit card (Diners Club, MasterCard or VISA).
  • Strain data was added.
October 30, 2009
  • Transgenic lines expressing Kaede reporter gene in neural tissues are now in available.
    These lines are useful marker for studying development and physiology of neurons.
June 4, 2009 March 4, 2009 March 2, 2009 November 6, 2008
  • Ciona intestinalis web page was opened.