Hox5 (KH.S765.3)
Vector name |
pSPU6CiHox5sg1 pDR274CiHox5sg1 |
Target sequence (lower case: PAM) | GGCGACGACGGGTTAGGTAAcgg |
Position of target site | homeodomain |
Primers for detecting mutations | CGAGCAAGCGAACCAGGACAG CAGCACTAATAGAATGTTAGTCG |
CelI analysis | Hox5sg1-RNA-CelI.pdf |
Sequenced mutations | Hox5sg1-RNA-mutation.pdf |
Reference | Sasaki et al (2014) Development Growth and Differentiation 56, 499-510 |