CesA(cis) (KH.C7.211)
Vector name |
pHTBCiCesAAP2BSTALEN-L1 pHTBCiCesAAP2BSTALEN-R1 |
Target sequence (lower case: spacer) | GAGCAAGTCCAACACAGttttcgcctgcgggcaCCAGCGTCCGACTCATG (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | cis element |
Primers for mutation detection (5’to 3’) | ggggatccTAACAGGAATTTCAATCAGTTTTTCC ccctgcaGTTGTCATTTGAGCAATACTTGC |
CelI analysis | CiCesAAP2BS-CelI.pdf |
Sequenced mutations | CesA-RNA-mutation.pdf |
Reference | Sasakura et al (2016) Proc Biol Sci 283, pii: 20161712 |