CesA(cis) (KH.C7.211)


Vector name pHTBCiCesAAP2BSTALEN-L1
pHTBCiCesAAP2BSTALEN-R1
Target sequence (lower case: spacer) GAGCAAGTCCAACACAGttttcgcctgcgggcaCCAGCGTCCGACTCATG
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site cis element
Primers for mutation detection (5’to 3’) ggggatccTAACAGGAATTTCAATCAGTTTTTCC
ccctgcaGTTGTCATTTGAGCAATACTTGC
CelI analysis CiCesAAP2BS-CelI.pdf
Sequenced mutations CesA-RNA-mutation.pdf
Reference Sasakura et al (2016) Proc Biol Sci 283, pii: 20161712