Hox1 (KH.L171.16)

Vector name pEF1>Hox1 TALEN-L
pEF1>Hox1 TALEN-R
pTitf>Hox1 TALEN-L
pTitf>Hox1 TALEN-R
pFr3dTPO>Hox1 TALEN-L
pFr3dTPO>Hox1 TALEN-R
Target sequence (lower case: spacer) TTCACTACAAAACAGCTtaccgagcttgaaaaAGAGTTTCACTTCAAT
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site homeodomain
Primers for mutation detection (5’to 3’) GGAAAAGGTTTCCCATCTTACC
AGTTACCTGGGTTTCATTAAGG
CelI analysis Hox1-EF1-CelI.pdf
Sequenced mutations Hox1-mutation-seq.pdf
Reference Yoshida et al (2017) Development 144, 1629-1634