Hox10 (KH.L132.17)

Vector name pEF1>Hox10TALEN-L
pEF1>Hox10TALEN-R
pZip>Hox10TALEN-L
pZip>Hox10TALEN-R
Target sequence (lower case: spacer) ACACAAAATATCAACTtctggaacttgagaaAGAATTTCATTACAATC
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site homeodomain
Primers for mutation detection (5’to 3’) AAGCTGACCCAACGAAACATTG
ATCTAGCTACAACAATAGCAGC
CelI analysis Hox10-EF-CelI.pdf
Sequenced mutations Hox10-EF1-mutation.pdf
Reference Kawai et al (2015) Developmental Biology 403, 43-56