Hox12 (KH.C7.472)

Vector name pBSCiEpiI>mcherry-CiEF1a>TALENCiHox12L
pBSCiEpiI>mcherry-CiEF1a>TALENCiHox12R
pBSCiEpiI>mcherry-CiEpiI>TALENCiHox12L
pBSCiEpiI>mcherry-CiEpiI>TALENCiHox12R
pHTBHox12 TALEN L
pHTBHox12 TALEN R
pBSCiEpiImCherryCiTiTF1CiHox12 TALEN L
pBSCiEpiImCherryCiTiTF1CiHox12 TALEN R
Target sequence (lower case: spacer) ACACTAAGTACCAACTTtccgagctagaaAGAGAGTTCGGAGCGA
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site homeodomain
Primers for mutation detection (5’to 3’) TTGTAGCTCACGACCATGTAG
ATCTTCGTCCTCTACAGACTG
CelI analysis Hox12-EF-CelI.pdf
Sequenced mutations Hox12-EF1-mutation.pdf
Reference Treen et al (2014) Development 141, 481-487
Yoshida et al (2017) Development 144, 1629-1634