Hox4 (KH.C1.959)
Vector name |
pHTBTALENCiHox4L pHTBTALENCiHox4R |
Target sequence (lower case: spacer) | TGAACTGGAAAAGGAGTTccattataatagatACCTTACAAGAAGAAGA (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | homeodomain |
Primers for mutation detection (5’to 3’) | TAAGAGACCAAGAACCGCTTAC ATGCAAGTTTTAGAATGTGAGTC |
CelI analysis | Hox4-RNA-CelI.pdf |
Sequenced mutations | Hox4-RNA-mutation.pdf |
Reference | Yoshida et al (2014) Genesis 52, 431-439 |