Hox4 (Platinum ver) (KH.C1.959)

Vector name pEF1>Hox4 TALEN L::2A::mCherry
pEF1>Hox4 TALEN R::2A::mCherry
pCesA>Hox4 TALEN L
pCesA>Hox4 TALEN R
pNut>Hox4 TALEN L
pNut>Hox4 TALEN R
pTnI>Hox4 TALEN L
pTnI>Hox4 TALEN R
Target sequence (lower case: spacer) GAACTGGAAAAGGAGTTccattataatagatACCTTACAAGAAGAAG
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site homeodomain
Primers for mutation detection (5’to 3’) TAAGAGACCAAGAACCGCTTAC
ATGCAAGTTTTAGAATGTGAGTC
CelI analysis Hox4-Platinum-EF1-HMA.pdf
Sequenced mutations Hox4-platinum-mutation-seq.pdf
Reference Yoshida et al (2017) Dev Biol 423, 111-125