Hox4 (Platinum ver) (KH.C1.959)
Vector name |
pEF1>Hox4 TALEN L::2A::mCherry pEF1>Hox4 TALEN R::2A::mCherry pCesA>Hox4 TALEN L pCesA>Hox4 TALEN R pNut>Hox4 TALEN L pNut>Hox4 TALEN R pTnI>Hox4 TALEN L pTnI>Hox4 TALEN R |
Target sequence (lower case: spacer) | GAACTGGAAAAGGAGTTccattataatagatACCTTACAAGAAGAAG (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | homeodomain |
Primers for mutation detection (5’to 3’) | TAAGAGACCAAGAACCGCTTAC ATGCAAGTTTTAGAATGTGAGTC |
CelI analysis | Hox4-Platinum-EF1-HMA.pdf |
Sequenced mutations | Hox4-platinum-mutation-seq.pdf |
Reference | Yoshida et al (2017) Dev Biol 423, 111-125 |