Hox5 (KH.S765.3)

Vector name pHTBTALENCiHox5L
pHTBTALENCiHox5R
Target sequence (lower case: spacer) AGAGGTCGCCCATACTTtgtgtctcaccgAACGTCAGATCAAGAT
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site homeodomain
Primers for mutation detection (5’to 3’) CGAGCAAGCGAACCAGGACAG
CAGCACTAATAGAATGTTAGTCG
CelI analysis
Sequenced mutations
Reference Yoshida et al (2014) Genesis 52, 431-439