MMP-2/9/13 (KH.L76.4)

Vector name pEF1>MMP-2/9/13TALEN-L
pEF1>MMP-2/9/13TALEN-R
pZip>MMP-2/9/13TALEN-L
pZip>MMP-2/9/13TALEN-R
Target sequence (lower case: spacer) ACACACCAGACATGAGTccatgccgtgttcgtaACACATTCAAGAGAGC
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site 4th exon
Primers for mutation detection (5’to 3’) aagcgaacagaatacagcacac
atgacgacacaaaacggatctc
CelI analysis MMP2-EF-CelI.pdf
Sequenced mutations MMP-EF1-mutation.pdf
Reference Kawai et al (2015) Developmental Biology 403, 43-56