RAR (KH.C9.580)

Vector name pEF1>RAR TALEN-L
pEF1>RAR TALEN-R
pTitf>RAR TALEN-L
pTitf>RAR TALEN-R
Target sequence (lower case: spacer) TCTTTCGACGTAGTGTgcagaagaacatgcagtATACTTGTCATCGTAAC
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site 3rd exon
Primers for mutation detection (5’to 3’) taataacacttctgtgtcatcg
ttgcacacataaattcagccag
CelI analysis RAR-EF1-HMA.pdf
Sequenced mutations CiRAR-mutation-seq.pdf
Reference Yoshida et al (2017) Development 144, 1629-1634