Important Notice
New price will be applied from October 1st, 2019, mainly due to the change on tax in Japan.
A fee for strains will be charged. (Refer to the Price List for the charges.)
Payment must be made by credit card (Diners Club, MasterCard or VISA).
  Please read the " How to Pay with a Credit Card" about the detailed credit-card payment method.
MTA is valid for the academic year. (January 10th, 2012)

Resource information

Mutant

show all(5)

GFP expressed images

Marker

show all(34)

GFP expressed images

Enhancer detection

show all(57)

GFP expressed images

Jump starter/Mutator

show all(11)

GFP expressed images

Other

show all(5)

GFP expressed images

UAS/Gal4

show all(2)

GFP expressed images

MASK

show all(10)

GFP expressed images

Fixed Specimen

show all(1)

Genotyping

request page

Construct

show all(348)

Knock out

What's New

  • March, 2019
  • December, 2018
    • In Spring 2018, the method book that summarized transgenic technologies in ascidians has been published from Springer (Crick here for the webpage: https://www.springer.com/la/book/9789811075445). Many NBRP resources have been introduced in the book. The TALEN-based knockout vector that targets cellulose synthase gene is now available from NBRP! This knockout vector is used for improving WISH at the larval stage.
  • December, 2018
    • In April, 2018, The manuscript that describes the mechanism of the MASK method, the maternally expressed mRNA-specific knockdown in Ciona intestinalis, has been published. Many NBRP transgenic lines were used in this research. Click here for details.
  • September, 2018
    • The manuscript that shows the mechanisms of dopaminergic neuron differentiation in Ciona was published in Genes and Development. Wild type Ciona, NBRP plasmids, and the transgenic line expressing Kaede fluorescent protein specifically in dopamine neurons (Tg[MiCiTHK]2) were used in this study. Moreover, single cell transcriptome analysis was adopted in this study. Click here for details.
  • August, 2018
    • The manuscript that describes the mechanisms of placode and neural crest evolution was published in Nature. Wild type Ciona and plasmids of NBRP were used in this study. Click here for details.
  • May, 2018
  • March, 2018
  • January, 2018
    • We started to provide protocols of the experiments using NBRP resources. We hope the protocols will facilitate your researches with our materials!
    • Strain data was added.
  • December, 2017
  • November, 2017
    • The review about the mechanisms of adult organ/tissue formation and metamorphosis of ascidians was published. Many transgenic lines of NBRP are displayed in the article. Click here for details.
  • October, 2017
  • August, 2017
    • New categories, Fixed Specimen and Genotyping, were added to strain data.
    • Prices of resources were revised. (Refer to Price List for details)
    • We are now charging for Construct distribution. (Refer to Price List for details)
  • June, 2017
    • The axon trajectories of adult Ciona was described. In this report, NBRP the transgenic lines expressing Kaede in the neurons were used. Click here for details.
  • May, 2017
    • The functions of Hox1 in the thyroid-homologous organ (endostyle) were characterized in Ciona. In this report, TALENs for famous developmentally relevant genes like Otx, Hox1, RAR and Raldh2 were created. These TALENs are available from NBRP. Click here for details.
  • April, 2017
  • March, 2017
    • A novel technique to establish Ciona mutant lines has been established. This technique utilizes TALEN-based genome editing and germ cell regeneration. In this report, new vector series that can monitor TALEN expression with the aid of mCherry fluorescence were created, and these vectors are now available from NBRP. Click here for details.

About Ciona Intestinalis Transgenic line RESources

about
Fig: Ciona intestinalis

In addition to their unique evolutionary position as invertebrate chordates, ascidians provide a simple experimental system to investigate molecular mechanisms underlying development, reproduction, endocrinology, and physiology.read more

Contact Information

Shimoda Marine Research Center, University of Tsukuba
Yasunori Sasakura