CesA(ORF) (KH.C7.211)
Vector name |
pFOG>CesA TALEN L1 pFOG>CesA TALEN R1 pHTB-CesA TALEN L1 pHTB-CesA TALEN R1 pEF1>CesA TALEN L1 pEF1>CesA TALEN R1 |
Target sequence (lower case: spacer) | TACGCAGAGCAGGAAT gtgtaatcatctttg ACGCAGATTTCATGCCA (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | 11th exon |
Primers for mutation detection (5’to 3’) | GGTGTCTATTTGCATTACGTCG GGTAAAATAGTCCTAAGCACAC |
CelI analysis | HMA-CesA-ORF.pdf |
Sequenced mutations | CesA-genome-mutations.pdf |
Reference | Yoshida and Treen (2018) Adv Exp Med Biol 1029, pp131-140 |