GABAARα (KY21.Chr9.573)

Vector name pEF1>GABAARα TALEN L1
pEF1>GABAARα TALEN R1
pHTB>GABAARα TALEN L1
pHTB>GABAARα TALEN R1
Target sequence (lower case: spacer) AACAGTTCCCAACAGATtactacgaatagatccgAATGGCAACATCCTGTA
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site 4th exon
Primers for mutation detection (5’to 3’) ctcaacaacaatatggtgcaac
atcctaaagagagtgttgttac
CelI analysis HMA-GABAARα.pdf
Sequenced mutations GABAARα-genome-mutations.pdf
Reference Hozumi A et al (2020) Curr Biol 30(8),1555-1561.e4