GABABR1 (KY21.Chr9.470)
Vector name |
pEF1>GABABR1 TALEN L1 pEF1>GABABR1 TALEN R1 pHTB>GABABR1 TALEN L1 pHTB>GABABR1 TALEN R1 |
Target sequence (lower case: spacer) | TTTGGTTGCTTGCTGCTgttttatcgagtatctcATTATCAGTTGCTACAA (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | Initiation codon |
Primers for mutation detection (5’to 3’) | agaaagtcagtcacgcagtg tcttacgaatccatagaccg |
CelI analysis | HMA-GABABR1.pdf |
Sequenced mutations | GABABR1-genome-mutations.pdf |
Reference | Hozumi A et al (2020) Curr Biol 30(8),1555-1561.e4 |