GABABR1 (KY21.Chr9.470)

Vector name pEF1>GABABR1 TALEN L1
pEF1>GABABR1 TALEN R1
pHTB>GABABR1 TALEN L1
pHTB>GABABR1 TALEN R1
Target sequence (lower case: spacer) TTTGGTTGCTTGCTGCTgttttatcgagtatctcATTATCAGTTGCTACAA
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site Initiation codon
Primers for mutation detection (5’to 3’) agaaagtcagtcacgcagtg
tcttacgaatccatagaccg
CelI analysis HMA-GABABR1.pdf
Sequenced mutations GABABR1-genome-mutations.pdf
Reference Hozumi A et al (2020) Curr Biol 30(8),1555-1561.e4