Hox10 (KH.L132.17)
Vector name |
pEF1>Hox10TALEN-L pEF1>Hox10TALEN-R pZip>Hox10TALEN-L pZip>Hox10TALEN-R |
Target sequence (lower case: spacer) | ACACAAAATATCAACTtctggaacttgagaaAGAATTTCATTACAATC (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | homeodomain |
Primers for mutation detection (5’to 3’) | AAGCTGACCCAACGAAACATTG ATCTAGCTACAACAATAGCAGC |
CelI analysis | Hox10-EF-CelI.pdf |
Sequenced mutations | Hox10-EF1-mutation.pdf |
Reference | Kawai et al (2015) Developmental Biology 403, 43-56 |