Otx (KH.C4.84)

Vector name pEF1>Otx TALEN-L2
pEF1>Otx TALEN-R2
pTitf>Otx TALEN-L
pTitf>Otx TALEN-R
Target sequence (lower case: spacer) CGGAAAGACAAGATATcccgatatctttatgAGAGAAGAAGTTGCCCT
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site homeodomain
Primers for mutation detection (5’to 3’) AAGTTGGCAATTTTGTTATGAAC
CAATATGCAAGCTTGAGGTTCG
CelI analysis Otx-LR2-EF1-CelI.pdf
Sequenced mutations Otx-mutation-seq.pdf
Reference Yoshida et al (2017) Development 144, 1629-1634