PC2 (KY21.Chr10.367)

Vector name pEF1>PC2 TALEN L1
pEF1>PC2 TALEN R1
pHTB>PC2 TALEN L1
pHTB>PC2 TALEN R1
Target sequence (lower case: spacer) TTTTTCAGAATAACACTggacaagccggtggaaaACCCGGTTTAGATTTAA
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site 4th exon
Primers for mutation detection (5’to 3’) gcggcataagacatttgagt
cgtgtcatcattccctttat
CelI analysis HMA-PC2.pdf
Sequenced mutations PC2-genome-mutations.pdf
Reference Hozumi A et al (2020) Curr Biol 30(8),1555-1561.e4