PC2 (KY21.Chr10.367)
Vector name |
pEF1>PC2 TALEN L1 pEF1>PC2 TALEN R1 pHTB>PC2 TALEN L1 pHTB>PC2 TALEN R1 |
Target sequence (lower case: spacer) | TTTTTCAGAATAACACTggacaagccggtggaaaACCCGGTTTAGATTTAA (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | 4th exon |
Primers for mutation detection (5’to 3’) | gcggcataagacatttgagt cgtgtcatcattccctttat |
CelI analysis | HMA-PC2.pdf |
Sequenced mutations | PC2-genome-mutations.pdf |
Reference | Hozumi A et al (2020) Curr Biol 30(8),1555-1561.e4 |