VP (KY21.Chr6.340)
Vector name |
pEF1>VP TALEN L1 pEF1>VP TALEN R1 pTnI>VP TALEN L1 pTnI>VP TALEN R1 |
Target sequence (lower case: spacer) | TTGGACGCGTGTTTTttccgggactgctctaACATGGATTGGTACCGC (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | 1st exon |
Primers for mutation detection (5’to 3’) | AGATACAGACTGTATAGATTTC AACCTGAAATGGTCTGTTATCC |
CelI analysis | HMA-VP.pdf |
Sequenced mutations | CiVp-genome-mutations.pdf |
Reference | Kawada et al (2021) Frontiers in Endocrinology 12:668564 |