gnrh1 ver2 (KY21.Chr1.1655)
Vector name |
pEF1>gnrh1 TALEN L2 pEF1>gnrh1 TALEN R2 pHTB>gnrh1 TALEN L2 pHTB>gnrh1 TALEN R2 |
Target sequence (lower case: spacer) | AGCGGGAAATTCGACTctctaaagctgcagaatAATTTCCCTCATCTCG (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | ORF |
Primers for mutation detection (5’to 3’) | acttcacaaatgttggatatcg gttggcttgcttgtatctgttc |
CelI analysis | HMA-gnrh1-2.pdf |
Sequenced mutations | gnrh1-2-genome-mutations.pdf |
Reference | Hozumi A et al (2020) Curr Biol 30(8),1555-1561.e4 |