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Author
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Title
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Journal
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Volume
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Pages
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Year
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Related resources
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| Chacha PP, Horie R, Kusakabe TG, Sasakura Y, Singh M, Horie T, Levine M. |
Neuronal identities derived by misexpression of the POU IV sensory determinant in a protovertebrate.
|
Proc Natl Acad Sci U S A |
119(4) |
|
2022 |
 Tg[MiCiPC2K]2
Tg[MiCiPC2K]3
|
| Kawada T, Shiraishi A, Matsubara S, Hozumi A, Horie T, Sasakura Y, Satake H. |
Vasopressin Promoter Transgenic and Vasopressin Gene-Edited Ascidian, Ciona intestinalis Type A (Ciona robusta): Innervation, Gene Expression Profiles, and Phenotypes.
|
Front Endocrinol (Lausanne) |
12() |
668564 |
2021 |
Tg[MiCiVPV]1
 pMiDestF
pBSCiEpiI>mcherry-CiEF1a>TALEN-NG
pTnI>TALEN-NG::2A::mCherry
pEF1>VP TALEN L1
pEF1>VP TALEN R1
pTnI>VP TALEN L1
pTnI>VP TALEN R1
pMiDestF-CiVP 5'- UTR-Venus
pSP-CiVP 5'-UTR-Venus
pSPVenus
|
| Yamazaki A, Yamakawa S, Morino Y, Sasakura Y, Wada H. |
Gene regulation of adult skeletogenesis in starfish and modifications during gene network co-option.
|
Sci Rep |
11(1) |
20111 |
2021 |
pHTB-TALEN-NG::2A::mCherry
|
| Bostwick M, Smith EL, Borba C, Newman-Smith E, Guleria I, Kourakis MJ, Smith WC. |
Antagonistic Inhibitory Circuits Integrate Visual and Gravitactic Behaviors.
|
Curr Biol |
30(4) |
600-609.e2 |
2020 |
pSPCiVGLUTK
pMiCiVGLUT4.3K
|
| Hozumi A, Matsunobu S, Mita K, Treen N, Sugihara T, Horie T, Sakuma T, Yamamoto T, Shiraishi A, Hamada M, Satoh N, Sakurai K, Satake H, Sasakura Y. |
GABA-Induced GnRH Release Triggers Chordate Metamorphosis.
|
Curr Biol |
30(8) |
1555-1561.e4 |
2020 |
tail regression failed
gnrh1>Kaede
gnrh2>Kaede
GABABR2>H2B::mCherry
pEF1>GABABR1 TALEN L1
pEF1>GABABR1 TALEN R1
pEF1>GABAARα TALEN L1
pEF1>GABAARα TALEN R1
pEF1>GABAARβ TALEN L1
pEF1>GABAARβ TALEN R1
pEF1>PC2 TALEN L1
pEF1>PC2 TALEN R1
pEF1>gnrh2 TALEN L1
pEF1>gnrh2 TALEN R1
pEF1>gnrh1 TALEN L1
pEF1>gnrh1 TALEN R1
pEF1>gnrh1 TALEN L2
pEF1>gnrh1 TALEN R2
pEF1>gnrh1 TALEN L3
pEF1>gnrh1 TALEN R3
pHTB>GABABR1 TALEN L1
pHTB>GABABR1 TALEN R1
pHTB>GABAARα TALEN L1
pHTB>GABAARα TALEN R1
pHTB>GABAARβ TALEN L1
pHTB>GABAARβ TALEN R1
pHTB>PC2 TALEN L1
pHTB>PC2 TALEN R1
pHTB>gnrh2 TALEN L1
pHTB>gnrh2 TALEN R1
pHTB>gnrh1 TALEN L1
pHTB>gnrh1 TALEN R1
pHTB>gnrh1 TALEN L2
pHTB>gnrh1 TALEN R2
pHTB>gnrh1 TALEN L3
pHTB>gnrh1 TALEN R3
|
| Osugi T, Sasakura Y, Satake H. |
The ventral peptidergic system of the adult ascidian Ciona robusta (Ciona intestinalis Type A) insights from a transgenic animal model.
|
Sci Rep |
10(1) |
1892 |
2020 |
Tg[MiCiPC2K]2
Tg[MiCiPC2K]3
|
| Tajima Y, Hozumi A, Yoshida K, Treen N, Sakuma T, Yamamoto T, Sasakura Y. |
Hox13 is essential for formation of a sensory organ at the terminal end of the sperm duct in Ciona.
|
Dev. Biol. |
458(1) |
120-131 |
2020 |
Tg[MiCiHox13K]1
Tg[MiCiHox13K]2
pMiCiHox13>Kaede (+intron)
pSPCiHox13>Kaede (Hox13>Kaede, +intron)
pSPNut>mCherry::2A::MiTP
pSPTnI>mCherry::2A::MiTP
pEF1>Hox13 TALEN L1
pEF1>Hox13 TALEN R1
pEF1>Hox13 TALEN HD3L
pEF1>Hox13 TALEN HD3R
pMesp>TALEN-NG::2A::mCherry (pBSMesp>Hox13TALEN::2A::mCherry)
pTitf1>Hox13 TALEN L1
pTitf1>Hox13 TALEN R1
pAKR>Hox13 TALEN L1
pAKR>Hox13 TALEN R1
pCesA>Hox13 TALEN L1
pCesA>Hox13 TALEN R1
pNut>Hox13 TALEN L1
pNut>Hox13 TALEN R1
pTnI>Hox13 TALEN L1
pTnI>Hox13 TALEN R1
pMesp>Hox13 TALEN L1
pMesp>Hox13 TALEN R1
pBSCi-Hox13
pBSCs-Hox13
pBSCi-PC2
pBSCs-PC2
pBSCs-EF1α
pNut>Hox13 TALEN HD3L
pNut>Hox13 TALEN HD3R
pHTBHox13 TALEN L1
pHTBHox13 TALEN R1
pHTBHox13 TALEN HD3L
pHTBHox13 TALEN HD3R
|
| Yamaji S, Hozumi A, Matsunobu S, Sasakura Y. |
Orchestration of the distinct morphogenetic movements in different tissues drives tail regression during ascidian metamorphosis.
|
Dev Biol |
465(1) |
66-78 |
2020 |
pEF1a>ROCK TALEN L
pEF1a>ROCK TALEN R
pFOG>ROCK TALEN L
pFOG>ROCK TALEN R
pSPCiCesAnKaede
pSPCiCesAVCAAX
pSPCiCesAnKaede;CiCesAVCAAX
pMiCiCesAnKaede;CiCesAVCAAX
|
| Cao C, Lemaire LA, Wang W, Yoon PH, Choi YA, Parsons LR, Matese JC, Wang W, Levine M, Chen K. |
Comprehensive single-cell transcriptome lineages of a proto-vertebrate.
|
Nature |
571(7765) |
349-354 |
2019 |
pSP-Kaede
|
| Horie R, Hazbun A, Chen K, Cao C, Levine M, Horie T. |
Shared evolutionary origin of vertebrate neural crest and cranial placodes.
|
Nature |
560(7717) |
228-232 |
2018 |
Tg[MiCiTHK]2
pSPeCFP-ter
pSPeCFP
|
| Horie T, Horie R, Chen K, Cao C, Nakagawa M, Kusakabe TG, Satoh N, Sasakura Y, Levine M. |
Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics.
|
Genes Dev. |
32(19-20) |
1297-1302 |
2018 |
Tg[MiCiTHK]2
pSPCiPtf1aCFP
pSPCiTHK
pSPCiβ2tubulinCiPtf1acDNA
pSPCiβ2tubulinCiMeiscDNA
pSPCiPtf1acDNA
pSPCiPtf1a ΔMO
pSPCiMeiscDNA
pSPCiGCYA2K
pSPCiGCYBK
pSPCiGnRHXK
pSPCiNtlBK
pSPCiPDE9aK
pSPPDEdK
pSPLectinK
pSPSS23A3K
pMiCiTHK
|
| Sasakura Y, Hozumi A. |
Formation of adult organs through metamorphosis in ascidians.
|
Wiley Interdiscip Rev Dev Biol |
7(2) |
|
2018 |
Tg[MiCiAKRK]1
E[MiTSAdTPOG]2
E[MiTSAdTPOG]6
E[MiTSAdTPOG]15
Tg[MiCiVACHTK]5
Tg[MiCiTnIG]3
Tg[MiCiPC2K]3
no gill slit(EJ[MiTSAdTPOG]124)
Tg[MiCiZipCiFkhK]1
Tg[MiCiVGLUT4.3K]2
Tg[MiCiHox12K]1
Hox12TAL2
pMiCiHox12K
pMiCiVGLUT4.3K
|
| Sasakura Y. |
The Enhancer Trap in Ciona.
|
Adv. Exp. Med. Biol. |
1029() |
121-129 |
2018 |
E[MiTSAdTPOG]2
E[MiTSAdTPOG]15
E[MiTSAdTPOG]19
E[MiTSAdTPOG]24
Mu[ISMiTSAdTPOG]1
EJ[MiTSAdTPOG]75
EJ[MiTSAdTPOG]87
|
| Sasakura Y. |
Germline Transgenesis in Ciona.
|
Adv. Exp. Med. Biol. |
1029() |
109-119 |
2018 |
swimming juvenile (Tg[MiTFr3dTPOG]45)
|
| Satoh T, Iitsuka T, Shiraishi A, Hozumi A, Satake H, Sasakura Y. |
piRNA-like small RNAs are responsible for the maternal-specific knockdown in the ascidian Ciona intestinalis Type A.
|
Sci Rep |
8(1) |
5869 |
2018 |
Tg[MiCiNutG]3
Tg[MiCiNutG]4
Tg[MiCiTnIGCipemG]2
Tg[MiCiTnIGCipemG]9
Tg[T2CiFr3dTPORCipemG]3
Tg[Fr3dTPORCipemG]4
Tg[MiFr3dTPORCipemK]5
Tg[MiFr3dTPORCipemwtG]3
Tg[MiFr3dTPORCipemwtG]4
Tg[MiFr3dTPORCipemwtG]6
Tg[MiCiTnIGCipemG]1
|
| Yoshida K, Treen N. |
TALEN-Based Knockout System.
|
Adv. Exp. Med. Biol. |
1029() |
131-139 |
2018 |
pFOG>CesA TALEN L1
pFOG>CesA TALEN R1
pHTB-CesA TALEN L1
pHTB-CesA TALEN R1
pEF1>CesA TALEN L1
pEF1>CesA TALEN R1
|
| Osugi T, Sasakura Y, Satake H. |
The nervous system of the adult ascidian Ciona intestinalis Type A (Ciona robusta): Insights from transgenic animal models.
|
PLoS ONE |
12(6) |
e0180227 |
2017 |
Tg[MiCiPC2K]2
Tg[MiCiPC2K]3
|
| Yoshida K, Hozumi A, Treen N, Sakuma T, Yamamoto T, Shirae-Kurabayashi M, Sasakura Y. |
Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells.
|
Dev. Biol. |
423 |
111-125 |
2017 |
Tg[MiCiTnIG]2
Tg[MiCiNutG]3
EJ[MiTSAdTPOG]75
EJ[MiTSAdTPOG]87
EJ[MiTSAdTPOG]101
Tg[MiCiTnIG]3
Hox12TAL2
pSPCiVACHTC
pEF1a>TALEN-NG::2A::mCherry
pAKR>TALEN-NG::2A::mCherry
pCesA>TALEN-NG::2A::mCherry
pEpiI>TALEN-NG::2A::mCherry
pNut>TALEN-NG::2A::mCherry
pTnI>TALEN-NG::2A::mCherry
pBSCiEpiImCherryCiTiTF1CiHox12 TALEN L
pBSCiEpiImCherryCiTiTF1CiHox12 TALEN R
pHTBHox12 TALEN L
pHTBHox12 TALEN R
pCesA>Hox4 TALEN L
pCesA>Hox4 TALEN R
pNut>Hox4 TALEN L
pNut>Hox4 TALEN R
pTnI>Hox4 TALEN L
pTnI>Hox4 TALEN R
pEF1>Hox4 TALEN L::2A::mCherry
pEF1>Hox4 TALEN R::2A::mCherry
|
| Yoshida K, Nakahata A, Treen N, Sakuma T, Yamamoto T, Sasakura Y. |
Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx.
|
Development |
144 |
1629-1634 |
2017 |
no gill slit(EJ[MiTSAdTPOG]124)
pTiTF1>TALEN-NG::2A::mCherry
pEF1>Hox1 TALEN-L
pEF1>Hox1 TALEN-R
pEF1>Otx TALEN-L2
pEF1>Otx TALEN-R2
pEF1>RAR TALEN-L
pEF1>RAR TALEN-R
pEF1>Raldh2 TALEN-L
pEF1>Raldh2 TALEN-R
pTitf1>Hox1
pTitf1>Otx
pMhc3>Kaede
pFr3dTPO>TALEN::2A::mCherry
pTitf>Hox1 TALEN-L
pTitf>Hox1 TALEN-R
pTitf>Otx TALEN-L
pTitf>Otx TALEN-R
pTitf>RAR TALEN-L
pTitf>RAR TALEN-R
pTitf>Raldh2 TALEN-L
pTitf>Raldh2 TALEN-R
pTnI>Raldh2 TALEN-L
pTnI>Raldh2 TALEN-R
pFr3dTPO>Hox1 TALEN-L
pFr3dTPO>Hox1 TALEN-R
|
| Ogura Y, Sasakura Y. |
Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation.
|
Dev Cell. |
37(2) |
148-61 |
2016 |
pSPCicdc25>Kaede
pSPCicdc25:CiFkh>Kaede
pHTB-Ciinte.AP2L2
pHTB-En::Ciinte.GATAa
pRN3-Ciinte.GATAb
pHTB-mVenus::PCNA
pHTB-Lifeact::Venus
pHTB-Ciinte.cdc25
|
| Sasakura Y, Ogura Y, Treen N, Yokomori R, Park SJ, Nakai K, Saiga H, Sakuma T, Yamamoto T, Fujiwara S, Yoshida K. |
Transcriptional regulation of a horizontally transferred gene from bacterium to chordate.
|
Proc. Biol. Sci. |
283(1845) |
pii: 20161712 |
2016 |
pSPCiEpiIG (= Ci-EpiI>GFP)
pSPdTPOG
pHTBCiCesAAP2BSTALEN-L1
pHTBCiCesAAP2BSTALEN-R1
pPDCiCesALacZ (upstream 2162bp)
pPDCsCesALacZ (upstream 1383bp)
pPDCsCesA533LacZ (up533)
pPDCsCesA444LacZ (up444)
pPDCiCesA450LacZ (up450)
pPDCiCesA554LacZ (up554)
Bra>Kaede
Ci-EpiI>LacZ
Ci-EpiI>Ci-AP2L2
pSP-Sgr4704-1(R)CiBrabp-Kaede (+S. griseus)
pSP-Sav5219-3(F)CiBrabp-Kaede (+S. avermi)
pSP-Scab57291-1(R)CiBrabp-Kaede (+S. scabies)
AP2BS+surr(F):Bra>Kaede
AP2BS+surr(R):Bra>Kaede
Ci-Fkh>LacZ
AP2BS+surr(F)+Ci-Fkh>LacZ
AP2BS+surr(R)+Ci-Fkh>LacZ
AP2BSsolo+Ci-Fkh>LacZ
AP2BS+surr(F)+Ci-TPO>GFP
AP2BS+surr(R)+Ci-TPO>GFP
AP2BSsolo+Ci-TPO>GFP
pSP-Sgr4704-1(F)CiFkhminimal-LacZ (+S. griseus)
pSP-Sav5219-3(R)CiFkhminimal-LacZ (+S. avermi)
pSP-Scab57291-1(F)CiFkhminimal-LacZ (+S. scabies)
|
| Hozumi A, Horie T, Sasakura Y. |
Neuronal map reveals the highly regionalized pattern of the juvenile central nervous system of the ascidian Ciona intestinalis.
|
Dev Dyn. |
244(11) |
1375-93 |
2015 |
Tg[MiCiVACHTK]5
Tg[MiCiβ2TBK]2
Tg[MiCiVGATK]1
Tg[MiCiVGATK]2
Tg[MiCiVGLUTK]5
Tg[MiCiVGLUTK]6
Tg[MiCib2TBC]3
Tg[MiCib2TBC]4
pSPCiβ2tubulin>CFP
pMiCiβ2tubulin>CFP
pSPChR2::YFP
pSPβ2tubulin>ChR2::YFP
pSPVACHT>ChR2::YFP
pSPVACHT>Kaede;VACHT>ChR2::YFP
|
| Kawai N, Ogura Y, Ikuta T, Saiga H, Hamada M, Sakuma T, Yamamoto T, Satoh N, Sasakura Y. |
Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine.
|
Dev Biol. |
403(1) |
43-56 |
2015 |
EJ[MiTSAdTPOG]107
Tg[MiCiZipCiFkhK]1
pTitf1-eCFP-Hox10
pBra-CFP-Hox10
pTitf1-Kaede
pSP-eCFP-Ci-Hox10
pZip-CFP-Hox10
pZip-VCAAX
pZip-H2B-mcherry
pSP-COL9-1A
pZip-COL9-1A
pHTB-Ci-Hox10sm
pHTB-Ci-Hox10
pEF1>Hox10TALEN-L
pEF1>Hox10TALEN-R
pZip>Hox10TALEN-L
pZip>Hox10TALEN-R
pEF1>MMP-2/9/13TALEN-L
pEF1>MMP-2/9/13TALEN-R
pZip>MMP-2/9/13TALEN-L
pZip>MMP-2/9/13TALEN-R
pBSCiEpiI>mcherry-CiZipCiFkh>TALEN-NG
|
| Iitsuka T, Mita K, Hozumi A, Hamada M, Satoh N, Sasakura Y. |
Transposon-mediated targeted and specific knockdown of maternally expressed transcripts in the ascidian Ciona intestinalis.
|
Sci Rep |
4() |
5050 |
2014 |
Tg[MiCiNutG]3
Tg[MiCiNutG]4
EJ[MiTSAdTPOG]78
Tg[MiCiTnIGCipemG]2
Tg[MiCiTnIGCipemG]9
Tg[MiCiTnIGCimTG]1
Tg[MiCiTnIGCipemG]1
pMiCiTnIGCipemG
pMiFr3dTPORCipemG
pMiCiTnIGCimTG
pMiCiTnIGCipem(-5'UTR)G
pMiFr3dTPORCipemCiNut(5'UTR)G
pMiCiTnIGCipemK
pRN3CipemFL
|
| Kamiya C, Ohta N, Ogura Y, Yoshida K, Horie T, Kusakabe TG, Satake H, Sasakura Y. |
Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis.
|
Dev. Dyn. |
243(12) |
1524-35 |
2014 |
|
| Kamiya C, Ohta N, Ogura Y, Yoshida K, Horie T, Kusakabe TG, Satake H, Sasakura Y. |
Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis.
|
Dev Dyn. |
243(12) |
1524-35 |
2014 |
Ciinte.Tg[pMi-Ciinte.EF1a>mKO2::hCdt1(1/100)]1
Ciinte.Tg[pMi-Ciinte.EF1a>mVeuns::hGem(1/110);Ciinte.EF1a>H2B::CFP]1
pMi-Ciinte.EF1a>mKO2::hCdt1(1/100)
pMi-Ciinte.EF1a>mVenus::hGem(1/110);Ciinte.EF1a>H2B::CFP
|
| Sasaki H, Yoshida K, Hozumi A, Sasakura Y. |
CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis.
|
Dev. Growth Differ. |
56(7) |
499-510 |
2014 |
pSPCiEF1a>Cas9
pHTBCas9
pSPU6CiHox3sg3
pSPU6CiHox5sg1
pDR274CiHox3sg1
pDR274CiHox3sg3
pDR274CiHox3sg4
pDR274CiHox3sg5
pDR274CiHox5sg1
pDR274CiHox12sg1
pDR274CiHox12sg2
pDR274CiHox12sg3
|
| Treen N, Yoshida K, Sakuma T, Sasaki H, Kawai N, Yamamoto T, Sasakura Y. |
Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona.
|
Development |
141(2) |
481-7 |
2014 |
pBSCiEpiI>mcherry-CiEF1a>TALEN-NG
pBSCiEpiI>Venus-CiEF1a>TALEN-NG
pBSCiEpiI>mcherry-CiEF1a>TALENCiFGF3L
pBSCiEpiI>Venus-CiEF1a>TALENCiFGF3R
pBSCiEpiI>mcherry-CiEF1a>TALENCiFGF11L
pBSCiEpiI>Venus-CiEF1a>TALENCiFGF11R
pBSCiEpiI>mcherry-CiEF1a>TALENCiHox12L
pBSCiEpiI>mcherry-CiEF1a>TALENCiHox12R
pBSCiEpiI>mcherry-CiEpiI>TALENCiFGF11L
pBSCiEpiI>mcherry-CiEpiI>TALENCiFGF11R
pBSCiEpiI>mcherry-CiEpiI>TALENCiHox12L
pBSCiEpiI>mcherry-CiEpiI>TALENCiHox12R
pBSCiEpiI>mcherry-CiNut>TALENCiFGF3L
pBSCiEpiI>mcherry-CiNut>TALENCiFGF3R
pBSCiEpiI>mcherry-CiNut>TALENCiFGF11L
pBSCiEpiI>mcherry-CiNut>TALENCiFGF11R
pBSCiEpiI>mcherry-CiPC2>TALENCiFGF3L
pBSCiEpiI>mcherry-CiPC2>TALENCiFGF3R
pBSCiEpiI>mcherry-CiPC2>TALENCiFGF11L
pBSCiEpiI>mcherry-CiPC2>TALENCiFGF11R
|
| Yokoyama TD, Hotta K, Oka K. |
Comprehensive morphological analysis of individual peripheral neuron dendritic arbors in ascidian larvae using the photoconvertible protein Kaede.
|
Dev Dyn. |
243(10) |
1362-73 |
2014 |
Tg[MiCiPC2K]2
Tg[MiCiPC2K]3
|
| Yoshida K, Treen N, Hozumi A, Sakuma T, Yamamoto T, Sasakura Y. |
Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases.
|
Genesis |
52(5) |
431-9 |
2014 |
pHTB-TAL4
pHTBTALENCiHox4L
pHTBTALENCiHox4R
pHTBTALENCiHox5L
pHTBTALENCiHox5R
|
| Hozumi A, Mita K, Miskey C, Mates L, Izsvak Z, Ivics Z, Satake H, Sasakura Y. |
Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element.
|
Dev. Dyn. |
242(1) |
30-43 |
2013 |
Tg[MiFr3dTPOCCiPrmSB100x]1
Tg[MiFr3dTPOCCiPrmSB100x]2
Tg[MiFr3dTPOCCiNutSB100x]1
Tg[MiFr3dTPOCCiNutSB100x]2
Tg[T2dTPOG]1
Tg[T2dTPOG]3
pAmpMinosneo
pT2dTPOG
pT2tet
pT2tet(L)
pRN3SB11
pRN3SB100X
pMiFr3dTPOCDestF
pSPeCFP
pSPFr3dTPOC
pMiFr3dTPOC
pMiFr3dTPOCCiNutSB100X
pMiFr3dTPOCCiPrmSB100X
pLMCSeGFP
pLMCSSB100X
pLMCSSB10
pLMCSSB11
pLMCSMiTP
pLCiNutSB100X
pLCiNutSB10
pLCiNutSB11
pLCiNutMiTP
pLCiprmSB100X
|
| Hozumi A, Yoshida R, Horie T, Sakuma T, Yamamoto T, Sasakura Y. |
Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome.
|
Dev. Biol. |
375(1) |
79-91 |
2013 |
E[MiTSAdTPOG]15
pSPCiGALNT6r(up6k)K
pSPCiGALNT6r(up3k)K
pSPCiGALNT6r(up2k)K
pSPdTPOG
pSPCiSMPD3down4kdTPOG
pSPCiSMPD3down4k/3'del1kdTPOG
pSPCiSMPD3down4k/3'del2kdTPOG
pSPCiGALNT6r(up6k)mKO2
pSPCiGALNT6r(up6k)mCherry
pSPCiGALNT6r(up6k)MiTSAdTPOG(F)mKO2
pSPCiGALNT6r(up6k)MiTSAdTPOG(R)mKO2
pSPCiGALNT6r(up6k)dTPOG(F)mCherry
pSPCiGALNT6r(up6k)dTPOG(R)mCherry
pSPCiFkhbasalG
pSPCiGALNT6r(up6k)CiFkhGbasal(F)mCherry
pSPCiGALNT6r(up6k)CiFkhGbasal(R)mCherry
pSPEE1175dTPOG
pSPEE1175/5'del215dTPOG
pSPEE1175/3'del695dTPOG
pSPEE1175/3'del938dTPOG
pSPCiGALNT6r(up6k)/EEdel3'695bp/dTPOG(F)mCherry
pSPCiGALNT6r(up6k)/EEdel3'695bp/dTPOG(R)mCherry
pSPCiGALNT6r(up6k)/EEdel3'938bp/dTPOG(F)mCherry
pSPCiGALNT6r(up6k)/EEdel3'938bp/dTPOG(R)mCherry
pSPCiGALNT6r(up6k)/upEEdTPOG(F)mCherry
pSPCiGALNT6r(up6k)/upEEdTPOG(R)mCherry
pSPCiGALNT6r(up6k)5'del934dTPOG(F)mCherry
pSPCiGALNT6r(up6k)5'del934dTPOG(R)mCherry
pSPCiGALNT6r(up6k)5'del1805dTPOG(F)mCherry
pSPCiGALNT6r(up6k)5'del1805dTPOG(R)mCherry
pSPCiGALNT6r(up6k)5'del2035dTPOG(F)mCherry
pSPCiGALNT6r(up6k)5'del2035dTPOG(R)mCherry
pSPCiGALNT6r(up6k)5'del2319dTPOG(F)mCherry
pSPCiGALNT6r(up6k)5'del2319dTPOG(R)mCherry
pSPCiGALNT6r(EEonly)dTPOG(F)mCherry
pSPCiGALNT6r(EEonly)dTPOG(R)mCherry
pSPdTPOG(F)CiGALNT6r(up2k)mCherry
pSPdTPOG(R)CiGALNT6r(up2k)mCherry
pBSOSRE(F)EE(F)
pBSOSRE(F)EE(R)
pBSOSRE(R)EE(F)
pBSOSRE(R)EE(R)
pSPOSRE(F)EE(F)dTPOG(F)CiGALNT6r(up2k)mCherry
pSPOSRE(F)EE(F)dTPOG(R)CiGALNT6r(up2k)mCherry
pSPOSRE(R)EE(R)dTPOG(F)CiGALNT6r(up2k)mCherry
pSPOSRE(R)EE(R)dTPOG(R)CiGALNT6r(up2k)mCherry
pSPOSRE(R)EE(F)dTPOG(F)CiGALNT6r(up2k)mCherry
pSPOSRE(R)EE(F)dTPOG(R)CiGALNT6r(up2k)mCherry
pSPOSRE(F)EE(R)dTPOG(F)CiGALNT6r(up2k)mCherry
pSPOSRE(F)EE(R)dTPOG(R)CiGALNT6r(up2k)mCherry
pSPEE(R)OSRE(R)dTPOG(F)CiGALNT6r(up2k)mCherry
pSPEE(R)OSRE(R)dTPOG(R)CiGALNT6r(up2k)mCherry
pSPmCherry
pSPmKO2
|
| Nakazawa K, Yamazawa T, Moriyama Y, Ogura Y, Kawai N, Sasakura Y, Saiga H. |
Formation of the digestive tract in Ciona intestinalis includes two distinct morphogenic processes between its anterior and posterior parts.
|
Dev. Dyn. |
242(10) |
1172-83 |
2013 |
Tg[MiCiZipCiFkhK]1
pSPCiZipCifkhK
pMiCiZipCifkhK
|
| Kawai N, Ochiai H, Sakuma T, Yamada L, Sawada H, Yamamoto T, Sasakura Y. |
Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases.
|
Dev. Growth Differ. |
54(5) |
535-45 |
2012 |
Tg[MiCiTnIG]2
pHTBEGFPZFN-L
pHTBEGFPZFN-R
|
| Mita K, Kawai N, Rueckert S, Sasakura Y. |
Large-scale infection of the ascidian Ciona intestinalis by the gregarine Lankesteria ascidiae in an inland culture system.
|
Dis. Aquat. Org. |
101(3) |
185-95 |
2012 |
|
| Nishitsuji K, Horie T, Ichinose A, Sasakura Y, Yasuo H, Kusakabe TG. |
Cell lineage and cis-regulation for a unique GABAergic/glycinergic neuron type in the larval nerve cord of the ascidian Ciona intestinalis.
|
Dev. Growth Differ. |
54(2) |
177-86 |
2012 |
|
| Razy-Krajka F, Brown ER, Horie T, Callebert J, Sasakura Y, Joly JS, Kusakabe TG, Vernier P. |
Monoaminergic modulation of photoreception in ascidian: evidence for a proto-hypothalamo-retinal territory.
|
BMC Biol. |
10() |
45 |
2012 |
|
| Sasakura Y, Kanda M, Ikeda T, Horie T, Kawai N, Ogura Y, Yoshida R, Hozumi A, Satoh N, Fujiwara S. |
Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis.
|
Development |
139(12) |
2156-60 |
2012 |
Tg[MiCiTnIG]2
Tg[MiCiEpiIG]3
Tg[MiCiEpiIG]4
Tg[MiCiTnIGCiprmG]2
no gill slit(EJ[MiTSAdTPOG]124)
pRN3CiHox1
pSPeCFP-ter
pSPeCFPCiHox1
pSPCiCesAeCFPCiHox1
pSPCiAKReCFPCiHox1
pSPCiβ2TBeCFPCiHox1
pSPCiTTF1eCFPCiHox1
pBSCiHox1
|
| Sasakura Y, Mita K, Ogura Y, Horie T. |
Ascidians as excellent chordate models for studying the development of the nervous system during embryogenesis and metamorphosis.
|
Dev. Growth Differ. |
54(3) |
420-37 |
2012 |
|
| Yoshida R, Sasakura Y. |
Establishment of Enhancer Detection Lines Expressing GFP in the Gut of the Ascidian Ciona intestinalis.
|
Zool. Sci. |
29(1) |
11-20 |
2012 |
E[MiTSAdTPOG]1
EJ[MiTSAdTPOG]36-2
EJ[MiTSAdTPOG]47-2
Mu[MiTSAdTPOG]8
EJ[MiTSAdTPOG]71
EJ[MiTSAdTPOG]75
EJ[MiTSAdTPOG]87
EJ[MiTSAdTPOG]92
EJ[MiTSAdTPOG]101
EJ[MiTSAdTPOG]107
EJ[MiTSAdTPOG]123
|
| Horie T, Shinki R, Ogura Y, Kusakabe TG, Satoh N, Sasakura Y. |
Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system.
|
Nature |
469(7331) |
525-8 |
2011 |
Tg[MiCiVACHTK]5
Tg[MiCiβ2TBK]2
Tg[MiCiVGATK]1
Tg[MiCiVGATK]2
pSPCiβ2TBK
pSPCiVGLUTK
pSPCiVGATK
pSPCiVACHTK
pSPCiopsin3K
pSPCiCRALBPK
pSPCiotpK
pSPCiPhox2K
pMiCiβ2TBK
pMiCiVGLUTK
pMiCiVGATK
pMiCiVACHTK
|
| Ogura Y, Sakaue-Sawano A, Nakagawa M, Satoh N, Miyawaki A, Sasakura Y. |
Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation.
|
Development |
138(3) |
577-87 |
2011 |
pcDNA3-CiEF1α-mKO2-hCdt1(1/100)
pcDNA3-CiEF1α-mKO2-hCdt1(30/120)
pT2-CiEF1α-mKO2-zCdt1(1/170)
pcDNA3-CiEF1α-mAG-hGem-CiEF1α-mKO2-hCdt1(1/100)
pcDNA3-CiEF1α-mAG-hGem-CiEF1α-mKO2-hCdt1(30/120)pMi-CiEF1α-mAG-hGem-CiEF1α-mKO2-hCdt1(30/120)
pT2-CiEF1α-mAG-hGem-CiEF1α-mKO2-zCdt1(1/170)
pSPGAP43C
pSPCiEpi1GAP43C
pSPKCAAX
pSPVCAAX
pSPmCheCAAX
pSPCiEpi1KCAAX
pSPCiETRmCheCAAX
pSPCiEpi1Cicdc25
pSPCiEpi1Cicdc25CiEpi1GAP43C
pSPCiEpi1Cicdc25CiEpi1VCAAX
pSPCiEpi1Cicdc25CiEpi1KCAAX
pSPCiEpi1Cicdc25CiEpi1mCheCAAX
pSPCiEpi1H2BmCherry
pBSHTBmAG-hGem
pBSHTBmKO2-hCdt1(1/100)
pBSHTBH2BmCherry
|
| Auger H, Sasakura Y, Joly JS, Jeffery WR. |
Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis.
|
Dev. Biol. |
339(2) |
374-89 |
2010 |
E[MiTSAdTPOG]15
|
| Hozumi A, Kawai N, Yoshida R, Ogura Y, Ohta N, Satake H, Satoh N, Sasakura Y. |
Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs.
|
Dev. Dyn. |
239(4) |
1076-88 |
2010 |
Tg[MiCiAKRK]1
Ju[SBFr3dTPORCiNutMiTP]1
Tg[MiCiTnIG]3
E[MiTSAdTPOG]25
E[MiTSAdTPOG]26
E[MiTSAdTPOG]27
pSP-Kaede
pSPCiNutMiTP
pSBFr3dTPORCiNutMiTP
pSPCiAKRK
pMiCiAKRK
|
| Ikuta T, Satoh N, Saiga H. |
Limited functions of Hox genes in the larval development of the ascidian Ciona intestinalis.
|
Development |
137(9) |
1505-13 |
2010 |
no gill slit(EJ[MiTSAdTPOG]124)
|
| Ohta N, Horie T, Satoh N, Sasakura Y. |
Transposon-mediated enhancer detection reveals the location, morphology and development of the cupular organs, which are putative hydrodynamic sensors, in the ascidian Ciona intestinalis.
|
Zool. Sci. |
27(11) |
842-50 |
2010 |
E[MiTSAdTPOG]10
EJ[MiTSAdTPOG]62
|
| Sasakura Y, Suzuki MM, Hozumi A, Inaba K, Satoh N. |
Maternal factor-mediated epigenetic gene silencing in the ascidian Ciona intestinalis.
|
Mol. Genet. Genomics |
283(1) |
99-110 |
2010 |
Tg[MiCiTnIG]2
Tg[MiCiEpiIG]3
Tg[MiCiEpiIG]4
Tg[MiCiCesAG]1
Tg[MiCiBraG]1
Tg[MiCiBraG]2
Tg[MiCiNutG]3
Tg[MiCiNutG]4
Tg[MiCiTnIGCiprmG]2
Tg[MiCiTrlG]1
Tg[MiCiEF1aG]1
Mu[ISMiTSAdTPOG]1
Tg[MiCiCesACsCesA-CiEpiIG]4
Tg[MiTFr3dTPOG]117
swimming juvenile2 (Tg[MiTFr3dTPOG]153)
pMiDestF
pMiCiEf1aFG
pMiCiCesAG
pMiCiCesA(PstI)G
pMiCiCesACsCesA
pMiCiCesACsCesA-CiEpiIG
pSPCiTrlG
pMiCiTrlG
pHTBeGFP
|
| Dahlberg C, Auger H, Dupont S, Sasakura Y, Thorndyke M, Joly JS. |
Refining the Ciona intestinalis model of central nervous system regeneration.
|
PLoS ONE. |
4(2) |
e4458 |
2009 |
E[MiTSAdTPOG]15
|
| Nakayama-Ishimura A, Chambon JP, Horie T, Satoh N, Sasakura Y. |
Delineating metamorphic pathways in the ascidian Ciona intestinalis.
|
Dev Biol. |
326(2) |
357-67 |
2009 |
swimming juvenile (Tg[MiTFr3dTPOG]45)
tail regression failed
|
| Sasakura Y, Konno A, Mizuno K, Satoh N, Inaba K. |
Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos.
|
Dev Dyn. |
237(1) |
39-50 |
2008 |
Tg[MiCiTnIGCiprmG]2
Tg[MiCiTnIGCiprmG]3
EJ[MiTSAdTPOG]10
EJ[MiTSAdTPOG]17
EJ[MiTSAdTPOG]21-1
EJ[MiTSAdTPOG]21-2
EJ[MiTSAdTPOG]24
EJ[MiTSAdTPOG]35
EJ[MiTSAdTPOG]36-2
EJ[MiTSAdTPOG]36-3
EJ[MiTSAdTPOG]38
EJ[MiTSAdTPOG]41
EJ[MiTSAdTPOG]42
EJ[MiTSAdTPOG]47-1
EJ[MiTSAdTPOG]47-2
Ju[SBFr3dTPORCiprmMiTP]1
Ju[SBFr3dTPORCiprmMiTP]2
pMiCiTnIG
pSPnDsRed
pSPCiprmG
pSPCiprmMiTP
pMiCiTnIGCiprmG
pSPFr3dTPOR
pSBFr3dTPOR
pSBFr3dTPORDestF
pSBFr3dTPORCiprmMiTP
pSPMiTP
|
