Strain Detail
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| Name | swimming juvenile (Tg[MiTFr3dTPOG]45) | |
| Type | Mutant | |
| Vector | pMiTFr3dTPOG | |
| transgene (derived organism) |
• Minos (Drosophila hydei)
• pBluescript (plasmid vector) • GFP (Aequorea victoria) • SV40 polyadenylation sequence (Papovavirus SV40) • nuclear localization sequence (Papovavirus SV40) • Transcription termination sequence of hsp70 gene (Drosophila melanogaster) |
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| Created Method | DNA/RNA microinjection | |
| Expression at the larval stage | no expression | |
| Expression at the juvenile stage | endostyle, peripharyngeal band, retropharyngeal band | |
| Expression at the adult stage | endostyle, peripharyngeal band, retropharyngeal band | |
| Picture at the larval stage |
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| Picture at the juvenile stage |
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| Picture at the adult stage | ||
| Movie | ||
| Phenotypes | defect in metamorphosis and tunic formation | |
| Sequence of insertion site | ||
| Gene present at the insertion site | Ci-CesA | |
| Reference |
Sasakura Y et al., Transposon-mediated insertional mutagenesis revealed the functions of animal cellulose synthase in the ascidian Ciona intestinalis., Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15134-9. Epub 2005 Oct 7.
Nakayama et al,Delineating metamorphic pathways in the ascidian Ciona intestinalis.,Dev Biol.,2009 Feb;326(2):357-67. Sasakura Y et al (2018) Adv. Exp. Med. Biol. 1029(),109-119 |
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| Available | sperm | |
| juvenile | ||
| adult | ||