Strain Detail

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Name swimming juvenile (Tg[MiTFr3dTPOG]45)
Type Mutant
Vector pMiTFr3dTPOG
transgene (derived organism)  • pBluescript (plasmid vector)
 • GFP (Aequorea victoria)
 • Transcription termination sequence of hsp70 gene (Drosophila melanogaster)
 • nuclear localization sequence (Papovavirus SV40)
 • Minos (Drosophila hydei)
 • SV40 polyadenylation sequence (Papovavirus SV40)
Created Method DNA/RNA microinjection
Expression at the larval stage no expression
Expression at the juvenile stage endostyle, peripharyngeal band, retropharyngeal band
Expression at the adult stage endostyle, peripharyngeal band, retropharyngeal band
Picture at the larval stage
Picture at the juvenile stage
Picture at the adult stage
Phenotypes defect in metamorphosis and tunic formation
Sequence of insertion site
Gene present at the insertion site Ci-CesA
Reference Sasakura Y et al., Transposon-mediated insertional mutagenesis revealed the functions of animal cellulose synthase in the ascidian Ciona intestinalis., Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15134-9. Epub 2005 Oct 7.

Nakayama et al,Delineating metamorphic pathways in the ascidian Ciona intestinalis.,Dev Biol.,2009 Feb;326(2):357-67.

Sasakura Y et al (2018) Adv. Exp. Med. Biol. 1029(),109-119

Available sperm