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|transgene (derived organism)||
• pBluescript (plasmid vector)
• GFP (Aequorea victoria)
• nuclear localization sequence (Papovavirus SV40)
• Minos (Drosophila hydei)
• SV40 polyadenylation sequence (Papovavirus SV40)
|Created Method||DNA/RNA electroporation|
|Expression at the larval stage||epidermis|
|Expression at the juvenile stage||epidermis|
|Expression at the adult stage|
|Picture at the larval stage|
|Picture at the juvenile stage|
|Picture at the adult stage|
|Sequence of insertion site|
|Gene present at the insertion site|
Joly JS et al., Culture of Ciona intestinalis in closed systems., Dev Dyn. 2007 Jul;236(7):1832-40.
Sasakura Y et al (2010) Mol. Genet. Genomics 283(1),99-110
Sasakura Y et al (2012) Development 139(12),2156-60