FGF11 (KH.L28.8)
Vector name |
pBSCiEpiI>mcherry-CiEF1a>TALENCiFGF11L pBSCiEpiI>mcherry-CiEF1a>TALENCiFGF11R pBSCiEpiI>mcherry-CiEpiI>TALENCiFGF11L pBSCiEpiI>mcherry-CiEpiI>TALENCiFGF11R pBSCiEpiI>mcherry-CiNut>TALENCiFGF11L pBSCiEpiI>mcherry-CiNut>TALENCiFGF11R pBSCiEpiI>mcherry-CiPC2>TALENCiFGF11L pBSCiEpiI>mcherry-CiPC2>TALENCiFGF11R |
Target sequence (lower case: spacer) | AAATATCTGGTGATTTTcgcacacggcaggttattAGTGTGCAGTATTCTGT (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | 5' UTR |
Primers for mutation detection (5’to 3’) | CAGCTATATAACGGGACAGATTTCCG CAGGAACAATAGCCGCACCTTTATG |
CelI analysis | FGF11-EF-CelI.pdf |
Sequenced mutations | FGF11-EF1-mutation.pdf |
Reference | Treen et al (2014) Development 141, 481-487 |