ROCK (KY21.Chr7.1048)

Vector name pEF1>ROCK TALEN L1
pEF1>ROCK TALEN R1
pFOG>ROCK TALEN L1
pFOG>ROCK TALEN R1
Target sequence (lower case: spacer) GGTGAAGTCCAACTAGTtcgtcacaaaccaactaAACGTGTATATGCCATG
(upper case indicates the TALEN binding sites, and lower case indicates spacer)
Position of target site 3rd exon
Primers for mutation detection (5’to 3’) ggtgctaccatctagaccta
gcacctgtgttttataccac
CelI analysis HMA-rock.pdf
Sequenced mutations ROCK-genome-mutations.pdf
Reference Yamaji et al (2020) Developmental Biology 465: 66-78