ROCK (KY21.Chr7.1048)
Vector name |
pEF1>ROCK TALEN L1 pEF1>ROCK TALEN R1 pFOG>ROCK TALEN L1 pFOG>ROCK TALEN R1 |
Target sequence (lower case: spacer) | GGTGAAGTCCAACTAGTtcgtcacaaaccaactaAACGTGTATATGCCATG (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | 3rd exon |
Primers for mutation detection (5’to 3’) | ggtgctaccatctagaccta gcacctgtgttttataccac |
CelI analysis | HMA-rock.pdf |
Sequenced mutations | ROCK-genome-mutations.pdf |
Reference | Yamaji et al (2020) Developmental Biology 465: 66-78 |