Strain Detail

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Name swimming juvenile (Tg[MiTFr3dTPOG]45)
Type Mutant
Vector pMiTFr3dTPOG
transgene (derived organism)  • Minos (Drosophila hydei)
 • pBluescript (plasmid vector)
 • GFP (Aequorea victoria)
 • SV40 polyadenylation sequence (Papovavirus SV40)
 • nuclear localization sequence (Papovavirus SV40)
 • Transcription termination sequence of hsp70 gene (Drosophila melanogaster)
Created Method DNA/RNA microinjection
Expression at the larval stage no expression
Expression at the juvenile stage endostyle, peripharyngeal band, retropharyngeal band
Expression at the adult stage endostyle, peripharyngeal band, retropharyngeal band
Picture at the larval stage
Picture at the juvenile stage
Picture at the adult stage
Phenotypes defect in metamorphosis and tunic formation
Sequence of insertion site
Gene present at the insertion site Ci-CesA
Reference Sasakura Y et al., Transposon-mediated insertional mutagenesis revealed the functions of animal cellulose synthase in the ascidian Ciona intestinalis., Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15134-9. Epub 2005 Oct 7.

Nakayama et al,Delineating metamorphic pathways in the ascidian Ciona intestinalis.,Dev Biol.,2009 Feb;326(2):357-67.

Sasakura Y et al (2018) Adv. Exp. Med. Biol. 1029(),109-119

Available sperm
juvenile
adult
 animal(s)