Otx (KH.C4.84)
Vector name |
pEF1>Otx TALEN-L2 pEF1>Otx TALEN-R2 pTitf>Otx TALEN-L pTitf>Otx TALEN-R |
Target sequence (lower case: spacer) | CGGAAAGACAAGATATcccgatatctttatgAGAGAAGAAGTTGCCCT (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | homeodomain |
Primers for mutation detection (5’to 3’) | AAGTTGGCAATTTTGTTATGAAC CAATATGCAAGCTTGAGGTTCG |
CelI analysis | Otx-LR2-EF1-CelI.pdf |
Sequenced mutations | Otx-mutation-seq.pdf |
Reference | Yoshida et al (2017) Development 144, 1629-1634 |