Hox5 (KH.S765.3)
Vector name |
pHTBTALENCiHox5L pHTBTALENCiHox5R |
Target sequence (lower case: spacer) | AGAGGTCGCCCATACTTtgtgtctcaccgAACGTCAGATCAAGAT (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | homeodomain |
Primers for mutation detection (5’to 3’) | CGAGCAAGCGAACCAGGACAG CAGCACTAATAGAATGTTAGTCG |
CelI analysis | |
Sequenced mutations | |
Reference | Yoshida et al (2014) Genesis 52, 431-439 |