Important Notice
The login method has been changed since January 28, 2020. See "How to Request".
New price will be applied from October 1st, 2019, mainly due to the change on tax in Japan.
A fee for strains will be charged. (Refer to the Price List for the charges.)
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  Please read the " How to Pay with a Credit Card" about the detailed credit-card payment method.
MTA is valid for the academic year. (January 10th, 2012)

Resource information


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GFP expressed images


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GFP expressed images

Enhancer detection

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GFP expressed images

Jump starter/Mutator

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GFP expressed images


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GFP expressed images


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GFP expressed images


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GFP expressed images

Fixed Specimen

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request page


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GFP expressed images


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Knock out

What's New

  • March, 2024
    • The following vectors have been added to our project. We look forward to your request!
    • Expression vectors for the transcription factor POUIV (or Pou4f).
    • Reporter constructs having the basal promoter of friend of GATA (or ZFPM) gene.
    • Codon optimized transposase cDNA of Minos.
  • December, 2023
    • The review article about the functions of nervous systems and hormones in the metamorphosis and adults of Ciona has been published. Photographs of NBRP marker lines of nervous systems are used. Click here for details.
  • November, 2023
    • The manuscript about the functions of Ciona CatSper protein in the sperm has been published. In this manuscript, CatSper was knocked out by Crispr/Cas9. Click here for details.
  • November, 2023
    • The manuscript describing the migration of the mesenchymal cells into the tunic during metamorphosis has been published. These mesenchymal cells become tunic cells at the adult stage. In this manuscript, the transgenic line expressing Kaede in the mesenchyme was used. Click here for details.
  • March, 2023
    • The manuscript investigating the regulation of the cleavage number during Ciona embryogenesis has been published. NBRP plasmids were used to overexpress genes. Click here for details.
      New transgenic lines and plasmid vectors have been added in our project. We are waiting for your request.
    • Genome editing constructs for mutating a gene encoding serine racemase.
    • Expression vectors for dominant-negative Sar1.
    • Reporter constructs.

About Ciona Intestinalis Transgenic line RESources

Fig: Ciona intestinalis

In addition to their unique evolutionary position as invertebrate chordates, ascidians provide a simple experimental system to investigate molecular mechanisms underlying development, reproduction, endocrinology, and physiology. read more

Contact Information

Shimoda Marine Research Center, University of Tsukuba
Yasunori Sasakura