Notice: Suspension of Services Due to the Inspection work of NIG

For the inspection work of the institute, services on our website will be stopped during the period of time stated below. We apologize for the inconvenience and thank you for your understanding in this matter.

Fri. 12th Nov. 2021 16:00 - Sun. 14th Nov. 13:00 (JST)
Important Notice
The login method has been changed since January 28, 2020. See "How to Request".
New price will be applied from October 1st, 2019, mainly due to the change on tax in Japan.
A fee for strains will be charged. (Refer to the Price List for the charges.)
Payment must be made by credit card (Diners Club, MasterCard or VISA).
  Please read the " How to Pay with a Credit Card" about the detailed credit-card payment method.
MTA is valid for the academic year. (January 10th, 2012)

Resource information

Mutant

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GFP expressed images

Marker

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GFP expressed images

Enhancer detection

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GFP expressed images

Jump starter/Mutator

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GFP expressed images

Other

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GFP expressed images

UAS/Gal4

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GFP expressed images

MASK

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GFP expressed images

Fixed Specimen

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Genotyping

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Indicator

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GFP expressed images

Construct

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Knock out

What's New

(Archives)
  • July 2021
    • The method culturing Ciona in a small aquarium was added on Protocol page.
  • March, 2021
  • March, 2021
    • We have started offering of the transgenic lines expressing GCaMP8 in the nervous systems at both larval and adult stages. We are waiting for your request.
  • March, 2021
    • TALEN vectors that target Hox13 were deposited into our project. Moreover, the basic vectors that makes the fusion of an arbitral ORF and a fluorescent protein gene were also added.
  • September, 2020
    • The manuscript that describes morphogenetic movement of cells during tail regression in Ciona was published. In this manuscript, our vectors for knockout and expression of fluorescent proteins were used. This manuscript used the transgenic lines expressing Kaede photoconvertible protein in larval epidermis. This line will be deposited into NBRP soon. Click here for details.
  • April, 2020
    • The manuscript that describes neuronal circuit regulating metamorphosis of Ciona was published. The neurotransmitter GABA induces the whole events of metamorphosis, while gonadotropin-releasing hormone (GnRH) induces tail regression as a downstream factor of GABA. In this study, many TALEN and reporter gene expression vectors of this NBRP project were used. In addition, our mutant line, tail regression failed (trf) was analyzed, to find that the causative gene of this mutant encodes prohormone convertase 2 (PC2), which is the peptidase necessary for the maturation of peptide hormones. Click here for details.
  • March, 2020
  • February, 2020
    • The manuscript that describes adult peptidergic neurons in Ciona was published. In this manuscript, the NBRP transgenic lines expressing Kaede fluorescent protein in the neurons were used. Click here for details.
  • February, 2020
    • The manuscript that describes the role of Hox13 in the ascidian Ciona intestinalis has been published. Hox13 is essential for the formation of orange-pigmented organ at the terminus of spermiduct. In this study, many TALEN and reporter gene expression vectors of this NBRP project were used. In addition, the transgenic lines that recapitulate expression pattern of Hox13 has been deposited in the NBRP. Click here for details.
  • December, 2019
    • Ciona larva possesses less than 200 neurons. With the simple nervous system, the larva regulates their swimming behavior in response to light and gravity. The manuscript describing the mechanisms of the regulation was published in Current Biology. In this manuscript, NBRP plasmids that can label specific neuron types were used. Click here for details.
  • March, 2019
  • December, 2018
    • In Spring 2018, the method book that summarized transgenic technologies in ascidians has been published from Springer (Crick here for the webpage: https://www.springer.com/la/book/9789811075445). Many NBRP resources have been introduced in the book. The TALEN-based knockout vector that targets cellulose synthase gene is now available from NBRP! This knockout vector is used for improving WISH at the larval stage.
  • December, 2018
    • In April, 2018, The manuscript that describes the mechanism of the MASK method, the maternally expressed mRNA-specific knockdown in Ciona intestinalis, has been published. Many NBRP transgenic lines were used in this research. Click here for details.
  • September, 2018
    • The manuscript that shows the mechanisms of dopaminergic neuron differentiation in Ciona was published in Genes and Development. Wild type Ciona, NBRP plasmids, and the transgenic line expressing Kaede fluorescent protein specifically in dopamine neurons (Tg[MiCiTHK]2) were used in this study. Moreover, single cell transcriptome analysis was adopted in this study. Click here for details.
  • August, 2018
    • The manuscript that describes the mechanisms of placode and neural crest evolution was published in Nature. Wild type Ciona and plasmids of NBRP were used in this study. Click here for details.
  • May, 2018
  • March, 2018
  • January, 2018
    • We started to provide protocols of the experiments using NBRP resources. We hope the protocols will facilitate your researches with our materials!
    • Strain data was added.
  • December, 2017
  • November, 2017
    • The review about the mechanisms of adult organ/tissue formation and metamorphosis of ascidians was published. Many transgenic lines of NBRP are displayed in the article. Click here for details.
  • October, 2017
  • August, 2017
    • New categories, Fixed Specimen and Genotyping, were added to strain data.
    • Prices of resources were revised. (Refer to Price List for details)
    • We are now charging for Construct distribution. (Refer to Price List for details)
  • June, 2017
    • The axon trajectories of adult Ciona was described. In this report, NBRP the transgenic lines expressing Kaede in the neurons were used. Click here for details.
  • May, 2017
    • The functions of Hox1 in the thyroid-homologous organ (endostyle) were characterized in Ciona. In this report, TALENs for famous developmentally relevant genes like Otx, Hox1, RAR and Raldh2 were created. These TALENs are available from NBRP. Click here for details.
  • April, 2017
  • March, 2017
    • A novel technique to establish Ciona mutant lines has been established. This technique utilizes TALEN-based genome editing and germ cell regeneration. In this report, new vector series that can monitor TALEN expression with the aid of mCherry fluorescence were created, and these vectors are now available from NBRP. Click here for details.

About Ciona Intestinalis Transgenic line RESources

about
Fig: Ciona intestinalis

In addition to their unique evolutionary position as invertebrate chordates, ascidians provide a simple experimental system to investigate molecular mechanisms underlying development, reproduction, endocrinology, and physiology. read more

Contact Information

Shimoda Marine Research Center, University of Tsukuba
Yasunori Sasakura