| Important Notice |
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 New price will be applied from April 1st, 2025. Please check the new prices here. Please see here for details.
The login method has been changed since January 28, 2020. See "How to Request".
A fee for strains will be charged. (Refer to the Price List for the charges.)
Payment must be made by credit card (Diners Club, MasterCard or VISA).Please read the " How to Pay with a Credit Card" about the detailed credit-card payment method. MTA is valid for the academic year. (January 10th, 2012)
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Resource information
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Mutant
show all（9）
GFP expressed images
Construct
What's New
- February, 2026
- Strain data was added.
- Construct data was added.
- Publications page was updated.
- Transgenic lines expressing GCaMP8 in the whole body, epidermis, neurons, or endoderm. As the counterstaining, mCherry is localized to the nuclei of GCaMP8-expressing cells. These lines will be useful for imaging of Ca2+
- Knockout vectors of transcription factor genes (POU4, TTF1/Nkx2-1, FoxE, FoxD)
- Knockout vectors for a TRP channel gene (PKD2)
- Expression vectors for GCaMP8
- December, 2025
- The article describing the functions of Hox3 in heart formation has been published. NBRP Kaede transgenic lines and knockout vectors were used. Click here for details.
- November, 2025
- The review article regarding germ cell formation in tunicates has been published. Photographs of NBRP transgenic lines are used. Click here for details.
- August, 2025
- The article introducing Imaging MS technology in Ciona has been published. NBRP neuron transgenic lines were used for the analyses. Click here for details.
- June, 2025
- The signal transduction upon the initiation of Ciona metamorphosis has been published. The expression vectors of G-proteins used in this study will be beneficial for investigating physiological phenomena in ascidians and have been deposited in NBRP. Click here for details.
- April, 2025
- Prices of resources were revised. (Refer to the Price List for the charges.)
- March, 2025
- Strain data was added.
- Construct data was added.
- Publications page was updated.
- Transgenic lines expressing GCaMP8 in the whole body, epidermis, neurons, or endoderm. As the counterstaining, mCherry is localized to the nuclei of GCaMP8-expressing cells. These lines will be useful for imaging of Ca2+
- Knockout vectors of transcription factor genes (POU4, TTF1/Nkx2-1, FoxE, FoxD)
- Knockout vectors for a TRP channel gene (PKD2)
- Expression vectors for GCaMP8
- January, 2025
- The article describing the functions of Epi-1, the tunicate-specific gene expressing in the epidermis, has been published. The mutants of Epi-1 will be deposited to NBRP. NBRP epidermis-GFP lines are used. Click here for details.
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The following transgenic lines and plasmid constructs have been added to our project.
We look forward to your request!
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The following transgenic lines and plasmid constructs have been added to our project.
We look forward to your request!
About Ciona Intestinalis Transgenic line RESources
Fig: Ciona intestinalis |
In addition to their unique evolutionary position as invertebrate chordates, ascidians provide a simple experimental system to investigate molecular mechanisms underlying development, reproduction, endocrinology, and physiology.
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Contact Information
Shimoda Marine Research Center, University of Tsukuba
Yasunori Sasakura