RAR (KH.C9.580)
Vector name |
pEF1>RAR TALEN-L pEF1>RAR TALEN-R pTitf>RAR TALEN-L pTitf>RAR TALEN-R |
Target sequence (lower case: spacer) | TCTTTCGACGTAGTGTgcagaagaacatgcagtATACTTGTCATCGTAAC (upper case indicates the TALEN binding sites, and lower case indicates spacer) |
Position of target site | 3rd exon |
Primers for mutation detection (5’to 3’) | taataacacttctgtgtcatcg ttgcacacataaattcagccag |
CelI analysis | RAR-EF1-HMA.pdf |
Sequenced mutations | CiRAR-mutation-seq.pdf |
Reference | Yoshida et al (2017) Development 144, 1629-1634 |