Strain Detail

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Name Ju[SBFr3dTPORCiprmMiTP]2
Type Jump starter/Mutator
Vector pSBFr3dTPORCiprmMiTP
transgene (derived organism)  • Sleeping Beauty transposon (Salmonid)
 • DsRed (Discosoma striata)
 • Minos transposase (Drosophila hydei)
 • pBluescript (plasmid vector)
 • SV40 polyadenylation sequence (Papovavirus SV40)
 • nuclear localization sequence (Papovavirus SV40)
 • Gateway recombination sequence (lambda phage)
Created Method DNA electroporation
Expression at the larval stage no expression
Expression at the juvenile stage muscle, endostyle, peripharyngeal band, retropharyngeal band (RFP)
Expression at the adult stage muscle, endostyle, peripharyngeal band, retropharyngeal band (RFP) sperm (MiTP)
Picture at the larval stage
Picture at the juvenile stage
Picture at the adult stage
Sequence of insertion site
Gene present at the insertion site
Reference Sasakura Y et al., Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos., Dev Dyn. 2008 Jan;237(1):39-50.

Available sperm The living animals of this transgenic line is currently unavailable. Please contact Yasunori Sasakura (), if you wish to use the line for your experiment.